Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience
FOXP3 단백질을 인식하는 FJK-16s 클론의 FITC 결합 단일클론 항체로, 마우스 및 랫트 조절 T세포 분석에 적합합니다. Flow Cytometry 및 IHC(F)에서 검증되었으며, 498/517 nm 형광 특성을 가집니다. Foxp3/Transcription Factor Buffer Staining Set과 함께 사용 권장.
- 카탈로그번호
- 11-5773-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience
Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 10 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 211 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Cat, Human, Mouse, Pig, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465242 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, Biotin
- Brilliant Violet 421, 605, 711, 786
- eFluor 450, 506, 570, 615, 660
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 is a 49–55 kDa transcriptional regulator identified as the gene defective in scurfy (sf) mice. It is highly expressed in CD4⁺CD25⁺ regulatory T cells (Treg), and ectopic expression in CD4⁺CD25⁻ cells induces a Treg phenotype.
Epitope mapping shows recognition of amino acids 75–125 of mouse Foxp3. This region is alternatively spliced in humans. FJK-16s is optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00). Use of other buffers is not recommended.
Applications Reported:
- Intracellular flow cytometry
- Immunohistochemistry of frozen tissue sections
Applications Tested:
Tested in intracellular flow cytometry of mouse splenocytes using Anti-Mouse Foxp3 FITC Staining Set (Product #71-5775-40).
Recommended ≤1 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue.
Filtered through 0.2 µm post-manufacturing.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is expressed in CD25⁺CD4⁺ regulatory T cells and absent in CD8⁺ T cells. FOXP3 defects cause the scurfy phenotype in mice and IPEX syndrome in humans. Transcript variants encoding different isoforms exist. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.