Thermo Fisher Scientific NME7 Polyclonal Antibody

Applications

• Immunocytochemistry (ICC/IF): Tested dilution 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
SIRALFGTDGI RNAAHGPDSF ASAAREMELF FPSSGGCGPA NTAKFTNCTC CIVKPHAVSE GLLGKILMAI RDAGFEISAM QMFNMDRVN

Highest antigen sequence identity to the following orthologs:

• Mouse: 87%
• Rat: 88%

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.

With more than 500 gene products, the protein kinase family is one of the largest protein families in eukaryotes. The family is classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) includes 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs), are well characterized in the yeast pheromone response pathway, where pheromones bind to Ste cell surface receptors and activate the yeast MAPK pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.