Thermo Fisher Scientific Phospho-AKT1 (Ser473, Tyr474) Polyclonal Antibody
AKT1 단백질의 Ser473 및 Tyr474 인산화 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체입니다. Western blot 및 Immunocytochemistry에 최적화되어 있으며, 인간, 마우스, 랫트 시료에 반응합니다. 액상 형태로 제공되며 고순도 정제 및 안정적 보관이 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1 (Accession P31749), corresponding to amino acid residues around phosphorylated Ser473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817471 |
Product Specific Information
This antibody detects endogenous levels of AKT1 only when phosphorylated at Ser473 and Tyr474.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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