Thermo Fisher Scientific Phospho-TSC2 (Ser1254) Polyclonal Antibody
TSC2 단백질의 Ser1254 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. PBS/glycerol 완충액에 보관하며 -20°C에서 저장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human TSC2 (Accession P49815), corresponding to amino acid residues around phosphorylated Ser1254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817470 |
Product Specific Information
This antibody detects endogenous levels of Tuberin/TSC2 only when phosphorylated at Ser1254.
Target Information
TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2) is a tumor suppressor protein involved in vesicular transport, regulation of cell growth arrest, and transcription mediated by steroid receptors. It forms a complex with hamartin (TSC1), acting as a chaperone and regulating cellular signaling through the PI3K/Akt pathway. Phosphorylation at Ser939 and Thr1462 modulates its interaction with hamartin. Mutations in TSC2 are associated with diseases such as lymphangioleiomyomatosis and angiomyolipoma. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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