Thermo Fisher Scientific Phospho-Aquaporin 2 (Ser261) Polyclonal Antibody
Phospho-Aquaporin 2 (Ser261) 특이적 검출용 폴리클로날 항체로, 인간·마우스·랫트 시료에서 사용 가능. Western blot에 최적화된 항체로, Ser261 인산화된 AQP2만 선택적으로 검출. 고순도 정제 및 안정적 PBS/glycerol 저장.
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Applications
Tested Application
- Western Blot (WB): 1:1,000–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AQP2 (Accession P41181), corresponding to amino acid residues around phosphorylated Ser261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817474 |
Product Specific Information
Antibody detects endogenous levels of AQP2 only when phosphorylated at Ser261.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. Vasopressin-induced cAMP increase results in phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Serine-261 has been identified as a novel phosphorylation site on AQP2, and phosphorylated S261 levels decrease with vasopressin treatment, suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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