Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Biotin, eBioscience
Human TNF-alpha를 인식하는 중화용 단일클론 항체로, Biotin 결합형. ELISA 검출용으로 최적화되어 있으며, 0.5–2 µg/mL 농도에서 사용 가능. IgG1, kappa 아이소타입이며, 4°C 암소 보관.
- 카탈로그번호
- 13-7349-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | - | View 24 publications |
| ELISA | 0.5–2 µg/mL | View 2 publications |
| Neutralization | - | View 1 publication |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466952 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
Description:
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4⁺ T cells. TNF-alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported:
Reported for use in detection of human TNF-alpha by ELISA.
Applications Tested:
Tested as a detection antibody in sandwich ELISA for human TNF-alpha analysis, in combination with purified MAb1 (14-7348) for capture and recombinant human TNF-alpha (14-8329) as standard. Recommended detection concentration: 0.5–2 µg/mL.
Include a standard curve of recombinant TNF-alpha with doubling dilutions from 1000 pg/mL to 8 pg/mL per ELISA plate.
Target Information
TNF-alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF-alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It forms multimers (dimers, trimers, pentamers) and shows a single 17 kDa band under non-reducing SDS-PAGE. Knockout studies in mice suggest neuroprotective functions and tumor necrosis activity. TNF-alpha is involved in immune responses to bacterial, viral, parasitic, and fungal infections, and contributes to tumor necrosis. It acts synergistically with interferon-gamma in cytotoxicity and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.