Thermo Fisher Scientific Phospho-iNOS (Tyr1055) Polyclonal Antibody
Phospho-iNOS (Tyr1055) 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot과 ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C 보관, 연구용 전용 제품입니다.
- 카탈로그번호
- PA5143731
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:300
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-iNOS (Tyr-1055) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Tyr-1055 in human iNOS. This sequence is well conserved in rat and mouse iNOS, and has some homology to the conserved sites in eNOS (Tyr-1087) and nNOS (Tyr-1326). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942959 |
Product Specific Information
The antibody detects a 130 kDa protein corresponding to induced NOS on SDS-PAGE immunoblots of mouse macrophages treated with LPS followed by pervanadate. It also detects a 150 kDa doublet corresponding to higher molecular weight forms of NOS, which are observed using other anti-iNOS antibodies. Additionally, a non-specific 120 kDa band may be detected.
Target Information
iNOS (Inducible Nitric oxide synthase) generates nitric oxide (NO), a gaseous free radical mediating vasorelaxation, neurotransmission, and cytotoxicity. iNOS is expressed in liver and inducible by lipopolysaccharide and cytokines. NOS family members catalyze oxidation of L-arginine to L-citrulline and NO, containing calmodulin, NADPH, FAD, and FMN binding domains. iNOS is found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokine stimulation increases iNOS mRNA, protein, and activity levels, leading to sustained NO production. iNOS dysfunction is associated with diseases such as achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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