Thermo Fisher Scientific Phospho-eNOS (Ser1177) Polyclonal Antibody
인간, 마우스, 랫트 eNOS의 Ser1177 인산화형을 검출하는 폴리클로날 항체. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 -20°C에서 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-eNOS (Ser-1177) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-1177 in human eNOS. This sequence is conserved in rat and mouse eNOS. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942958 |
Product Specific Information
The antibody detects a 140 kDa* band corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells grown normally or treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission, and cytotoxicity can all be potentiated through cellular response to NO.
NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidation of L-arginine to produce L-citrulline and NO.
Two constitutive isoforms—brain or neuronal NOS (b/nNOS, type I) and endothelial cell NOS (eNOS, type III)—and one inducible isoform (iNOS, type II) have been cloned.
All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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