Thermo Fisher Scientific Phospho-MYL12A (Ser19) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-MLC (Ser-19) synthetic peptide (coupled to KLH) corresponds to amino acid residues surrounding Ser-19 in human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in other smooth muscle and non-muscle MLCs.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942955

Product Specific Information

This antibody was cross-adsorbed to MLC (a.a. 11-22) then affinity purified using phospho-MLC (Ser-19) peptide (without carrier). The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF and in mouse C2C12 treated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment of MLC.

Target Information

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.