
Thermo Fisher Scientific CD31 Recombinant Rabbit Monoclonal Antibody (078)
인체 CD31/PECAM1 단백질을 인식하는 재조합 토끼 단일클론 항체입니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 높은 특이성과 일관된 품질을 제공합니다. 방부제 무첨가로 민감한 실험에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 078 |
| Immunogen | Recombinant Human CD31/PECAM1 protein (Met1–Lys601) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.22 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785333 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human CD31/PECAM1.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T and B cell signaling via immunoreceptor tyrosine-based inhibitory motifs (ITIM). Upon phosphorylation, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is widely expressed within the vascular compartment, mainly at junctions between adjacent cells. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
Functions include:
- Modulation of integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Roles in autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis
CD31 is a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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