Thermo Fisher Scientific Nanog Polyclonal Antibody
Nanog 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간 및 마우스 시료에 반응하며 WB, IHC, ICC/IF, ChIP 등 다양한 응용에 적합. 단백질 A로 정제된 액상 항체로, -20°C에서 안정적으로 보관 가능.
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Thermo Fisher Scientific Nanog Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 7 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 11 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
| ChIP assay (ChIP) | 1–3 µL | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length human recombinant Nanog protein expressed in bacteria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539867 |
Product Specific Information
This antibody is not recommended for mouse testes tissue or human ovarian carcinoma in IHC applications.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that regulates pluripotency and differentiation of embryonic stem cells.
- Expressed in pluripotent human and mouse cell lines, absent in differentiated cells.
- Shares 52% overall amino acid identity with mouse NANOG and 85% identity in the homeodomain.
- Human NANOG maps to chromosome locus 12p13.31; mouse NANOG maps to locus 6 F2.
- Plays a key role in the inner cell mass and embryonic stem (ES) cell proliferation and self-renewal.
- Overexpression promotes S-phase entry and proliferation.
- Associated diseases: teratocarcinoma and germ cell/embryonal cancer.
NANOG is also involved in the generation of induced pluripotent stem (iPS) cells, which may be useful in regenerative medicine. Expression of NANOG, Sox2, Klf4, and c-Myc can generate iPS cells without viral vectors, improving safety for clinical applications.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
제품 이미지
(이미지 파일: PA1-097_Nanog_Q9H9S0-1_Rabbit.svg, PA1-097_Nanog_Q9H9S0-1_Rabbit_PDP.jpeg)
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