Thermo Fisher Scientific NEFM Polyclonal Antibody
NEFM 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합하며 다양한 종에 반응. Chicken IgY 기반으로 제작되었으며 PBS 버퍼에 0.02% sodium azide 포함. 신경세포 마커 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Chicken, Horse, Human, Mouse, Pig, Rat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant construct containing the C-terminus of the human sequence (amino acids 708–877) expressed in and purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077186 |
Product Specific Information
PA1-10001 was made against a recombinant fusion protein of E. coli TrpE fused to the C-terminus of rat NF-M, amino acids 677–845.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, most prominently in large axons. They consist of three polypeptide chains—NF-M, NF-H, and NF-L—that share structural similarity but differ in terminal sequences. NF-M’s phosphorylated tail domain mediates filament interactions critical for axonal architecture. NF-M phosphorylation and O-GlcNAcylation are reciprocally regulated, influencing filament formation and function. Antibodies against NF subunits serve as stable, neuron-specific markers useful for neurobiological research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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