Thermo Fisher Scientific Vimentin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:5,000

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Full length recombinant human vimentin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vimentin,uniProtId:P08670-1,ncbiNodeId:9606,antigenRange:1-466,antigenLength:466,antigenImageFileName:PA1-10003_Vimentin_P08670-1_Chicken.svg,antigenImageFileNamePDP:PA1-10003_Vimentin_P08670-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2216267

Product Specific Information

PA1-10003 can be used to study stem cells and generally to reveal the intermediate filament cytoskeleton. The immunogen used to generate this antibody was full length recombinant human vimentin. The antibody works well on all mammals tested to date, and it was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.