Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 506, eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue & Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b forms a non-covalent complex with CD18 to generate Mac-1 (αMβ2 integrin), binding to CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 cross-reacts with human CD11b, enabling detection on human monocytes, granulocytes, and NK cell subsets.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry on mouse bone marrow cells (≤0.125 µg/test, 100 µL final volume)
• Recommended: Empirical titration for optimal assay performance

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm (Violet laser compatible).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that associates with CD18 to form Mac-1 (CR3).
Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
Functions as a receptor for ICAMs, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell adhesion and immune interactions.
CD11b expression varies by cell type and tissue, increasing during monocyte maturation and serving as a microglial marker.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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