Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), eFluor 450, eBioscience
인간 CD8a에 특이적인 단클론 항체로, eFluor 450 형광체 결합 제품입니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 405/445 nm 파장에서 작동합니다. T 세포 및 NK 세포 표지에 활용되며, 고순도 친화 크로마토그래피로 정제되었습니다.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 43 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T8 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272131 |
Additional Formats Available
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV / Violet series
- eFluor 506
- FITC
- NovaFluor Blue, Red, Yellow series
- PE and PE-Cyanine variants
- PerCP-Cyanine5.5
- Super Bright series
- Custom conjugation available upon request
Product Specific Information
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimer or CD8α/α homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and associates with p56lck, playing a key role in T-cell development and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test
- Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction and T-cell activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer (CD8α/β) functioning as a co-receptor for MHC-I. It is also found as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide binding, CD8 recruits Lck kinase, triggering lymphokine production and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs are crucial for pathogen and tumor clearance, with differentiation enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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