Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), eFluor 450, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV / Violet series
• eFluor 506
• FITC
• NovaFluor Blue, Red, Yellow series
• PE and PE-Cyanine variants
• PerCP-Cyanine5.5
• Super Bright series
• Custom conjugation available upon request

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimer or CD8α/α homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and associates with p56lck, playing a key role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test
• Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction and T-cell activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer (CD8α/β) functioning as a co-receptor for MHC-I. It is also found as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide binding, CD8 recruits Lck kinase, triggering lymphokine production and cytotoxic T lymphocyte (CTL) activation.

Activated CTLs are crucial for pathogen and tumor clearance, with differentiation enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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