Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), FITC, eBioscience

Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), FITC, eBioscience

Applications and Tested Dilution

• Immunohistochemistry (IHC): Not specified
  View 1 publication
• Flow Cytometry (Flow): 5 µL (0.125 µg)/test
  View 13 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450
• NovaFluor Blue/Red/Violet/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Request custom conjugation

Product Specific Information

The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5, also known as BLR1). CD185 is a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma.
In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (not Th1 or Th2), and a subset of memory (CD45RO+) T cells. CD185+ T cells migrate to lymph nodes due to CCR7 and CD62L expression.
In tonsil tissue, CD185 is expressed on most CD4+ cells along with activation markers (CD69, ICOS), and supports antibody production when co-cultured with B cells.
This receptor plays a key role in lymphocyte trafficking, particularly T cell migration into B cell follicles in germinal centers responding to CXCL13, marking follicular helper T cells.
Cross-reactivity has been observed with Rhesus macaque.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg) per test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185) or Burkitt lymphoma receptor 1 (BLR1) belongs to the CXC chemokine receptor family. It is a G protein-coupled receptor for chemokine BLC and is expressed mainly in lymphatic tissues such as lymph node follicles and spleen.
CXCR5 plays an essential role in B cell migration and is expressed in various human and animal tissues including blood, bone marrow, brain, liver, lymph node, spleen, and tonsil.
GPCRs share a seven-transmembrane structure and are pivotal in cell signaling. They are important pharmacological targets, with drugs such as Zyprexa and Claritin developed against GPCRs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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