Thermo Fisher Scientific HP1 alpha Monoclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Immunogen

Purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HP1 alpha,uniProtId:P45973-1,ncbiNodeId:9606,antigenRange:1-191,antigenLength:191,antigenImageFileName:MA1-218_HP1_alpha_P45973-1_House_mouse.svg,antigenImageFileNamePDP:MA1-218_HP1_alpha_P45973-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2723247

Product Specific Information

MA1-218 detects CBX5 from human and non-human primate samples. CBX5 has an approximate molecular weight of 22.2kDa.

MA1-218 has been successfully used in Western blot, immunofluorescence,and immunohistochemistry applications.

The MA1-218 Immunogen is the purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.

Target Information

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double- primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.