Thermo Fisher Scientific HP1 alpha Monoclonal Antibody
HP1 alpha 단백질을 표적하는 Thermo Fisher Scientific의 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인체 및 비인체 영장류 시료에서 CBX5 검출 가능. Protein A 정제, PBS buffer, sodium azide 포함.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Purified recombinant fragment of human CBX5 (AA: 1–191) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723247 |
Product Specific Information
MA1-218 detects CBX5 from human and non-human primate samples.
CBX5 has an approximate molecular weight of 22.2 kDa.
MA1-218 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry applications.
The MA1-218 Immunogen is the purified recombinant fragment of human CBX5 (AA: 1–191) expressed in E. coli.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.
Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins—a family of heterochromatic adaptor molecules implicated in gene silencing and chromatin structure.
High-affinity recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain, which serves as a specific interaction motif for the methyl epitope on lysine-9 of histone H3.
In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-primary mouse fibroblasts but restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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