Thermo Fisher Scientific CDK7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal, 0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552143

Product Specific Information

Recommended positive controls: 293T, A431, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.

Predicted reactivity: Mouse (95%), Zebrafish (85%), Xenopus laevis (86%), Pig (97%), Rhesus Monkey (99%), Bovine (96%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The trimeric complex of CDK7, cyclin H and MNAT1, functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This complex is thought to serve as a direct link between the regulation of transcription and the cell cycle. The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). CDK7 may be a target of germline cancer-inducing mutations.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.