Thermo Fisher Scientific ASAH2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Suggested positive control: 293 cell lysate
• PA5-20575 can be used with blocking peptide PEP-0695

Target Information

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity.
ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5–8.5.

ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine.

Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed, with higher levels in the intestine, kidney, skeletal muscle, and heart.

Recent studies indicate that ASAH2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.