Thermo Fisher Scientific NENF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (IHC)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide from near the carboxy terminus of human NENF. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NENF,uniProtId:Q9UMX5-1,ncbiNodeId:9606,antigenRange:172,antigenLength:172,antigenImageFileName:PA5-20577_NENF_Q9UMX5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20577_NENF_Q9UMX5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153616

Product Specific Information

Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE. A suggested positive control is human small intestine tissue lysate.

PA5-20577 can be used with blocking peptide PEP-0697.

Target Information

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.