Thermo Fisher Scientific IL-21 Monoclonal Antibody (FFA21), PE, eBioscience
마우스 IL-21을 인식하는 FFA21 단클론 항체로, PE 형광 표지되어 있습니다. Flow Cytometry 및 세포 내 염색에 최적화되어 있으며, eBioscience IC 고정·투과 키트와 호환됩니다. IL-21 관련 면역반응 연구 및 Th17 세포 분석에 적합합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-21 Monoclonal Antibody (FFA21), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FFA21 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834468 |
Additional Formats
Product Specific Information
This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21), a 17 kDa immunomodulatory cytokine produced mainly by NKT, Th17, and TFH cells.
In TFH cells, IL-21 expression leads to autocrine signaling through IL-21R and STAT3, activating Bcl6 transcription. IL-21 is crucial for TFH effector function and plays a role in B cell differentiation, IgG production, and apoptosis of naive B cells.
In Th17 cells, IL-21 expression with STAT3, IRF4, and RORγt upregulates IL-23R, promoting Th17 maturation and maintenance via IL-23. IL-21 also downregulates Foxp3. Elevated IL-21 levels are observed in colitis models, while IL-21-deficient mice are resistant to colitis induction.
Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (Product #51-7213-82).
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Optimization: Compatible with eBioscience IC Fixation and Permeabilization Kit (Product #88-8824-00).
Applications Tested: Tested on Th17-polarized mouse splenocytes restimulated with PMA, ionomycin, and brefeldin A for 5 hours. Recommended ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-21 is a 17 kDa cytokine primarily produced by NKT, Th17, and TFH cells. It mediates autocrine signaling via IL-21R and STAT3, activating Bcl6 and promoting TFH cell function. IL-21 facilitates T cell-dependent B cell differentiation, IgG production, and apoptosis in naive B cells.
In Th17 cells, IL-21 with STAT3, IRF4, and RORγt induces IL-23R expression, enabling Th17 maturation. IL-21 also suppresses Foxp3 expression. IL-21-deficient mice show resistance to chemically induced colitis, whereas elevated IL-21 levels are observed in colitis models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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