Thermo Fisher Scientific IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), PE, eBioscience
인간 IL-21을 인식하는 단클론 항체로, NK 및 T 세포 활성화 연구에 적합. PE 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. 4°C 암소 보관, 동결 금지. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
- View 20 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio3A3-N2 (3A3-N2) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582261 |
Additional Formats
Product Specific Information
Description:
Monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein related to IL-2 and IL-15, expressed in activated CD4+ cells. It promotes B-cell proliferation (via CD40 crosslinking), bone marrow progenitor cell proliferation, and NK-cell marker CD56 expression in the presence of IL-15.
Applications Reported:
Reported for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining of restimulated, Th17-polarized normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test. A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test, empirically determined).
Notes:
- Staining for IL-21 shows donor variability; stimulation times may vary and should be optimized.
- Increased staining levels observed on Th17-polarized normal human peripheral blood cells.
- Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer (NKT), T helper (Th)17, and T follicular helper (TFH) cells.
In TFH cells, IL-21 triggers autocrine signaling via IL-21R and STAT3, leading to Bcl6 activation. Like IFN-γ for Th1 and IL-4 for Th2, IL-21 is critical for TFH cell development and effector function.
IL-21 regulates T cell-dependent B cell differentiation into plasma and memory cells, stimulates IgG production, and induces apoptosis in naive B cells.
In Th17 cells, IL-21 enhances IL-23R expression via STAT3, IRF4, and RORγt, facilitating maturation and maintenance by IL-23. It also downregulates Foxp3.
IL-21 deficient mice are resistant to chemically induced colitis, while elevated IL-21 levels are seen in colitis models.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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