Thermo Fisher Scientific Doublecortin Monoclonal Antibody (2G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2G5

Immunogen

Purified recombinant fragment of human DCX (Uniprot: aa 281-335) expressed in E. Coli (NP_000546: aa 462-411). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Doublecortin,uniProtId:O43602-1,ncbiNodeId:9606,antigenRange:281-335,antigenLength:365,antigenImageFileName:MA5-17066_Doublecortin_O43602-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17066_Doublecortin_O43602-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538537

Product Specific Information

MA5-17066 targets DCX in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17066 immunogen is purified recombinant fragment of human DCX (amino acids: 362-411) expressed in E. Coli.

MA5-17066 detects DCX which has a predicted molecular weight of approximately 49.3kDa.

Target Information

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.