Thermo Fisher Scientific Human TRAIL (TNFSF10) Recombinant Protein
Human TRAIL (TNFSF10) 재조합 단백질로, E. coli에서 발현된 고순도(≥98%) 단백질입니다. 세포 사멸 연구 및 기능 분석(ELISA, WB, IHC 등)에 사용됩니다. Carrier-free 형태로 제공되며, 안정적인 장기 보관이 가능합니다.
- 카탈로그번호
- PHC1634
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Contains 168 amino acids |
| Molecular Weight | 19.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 1–3 ng/mL; determined by dose-dependent apoptosis of U34MG cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 10 mM sodium phosphate, pH 7.2, with 110 mM NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
- Reconstitution: Briefly centrifuge vial before opening. Reconstitute in sterile distilled water to 0.5–1.0 mg/mL. Do not vortex.
- Store reconstituted solution at 2–8°C for up to 1 week.
- For extended storage, dilute in buffer containing carrier protein (e.g., 0.1% BSA) and store aliquots at -20°C to -80°C.
- Storage: Lyophilized protein stable at room temperature for 1 month; for long-term storage, keep at -20°C, preferably desiccated.
- Working aliquots with carrier protein are stable ≥12 months at -20°C to -80°C.
- Avoid repeated freeze/thaw cycles.
Target Information
TRAIL (TNFSF10) is a cytokine belonging to the TNF ligand family. It preferentially induces apoptosis in transformed and tumor cells but not in normal cells, despite expression in most normal tissues.
TRAIL binds to several TNF receptor superfamily members, including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG.
Its activity may be modulated by decoy receptors (TRAILR3, TRAILR4, OPG) that do not induce apoptosis.
Binding of TRAIL to its receptors activates MAPK8/JNK, caspase 8, and caspase 3.
Receptors include death domain-containing DR4 and DR5, and decoy receptors DcR1 and DcR2.
TRAIL is a type II membrane protein expressed in various human tissues and induces apoptosis and NF-κB activation similar to TNF and Fas ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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