Thermo Fisher Scientific Human Noggin Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Functional Assay (Functional): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free

• Reconstitution:
  Centrifuge the vial briefly before opening to bring the contents to the bottom.
  Reconstitute the lyophilized protein in 10 mM acetic acid to a concentration of 0.1–1.0 mg/mL.
  Apportion the reconstituted protein into working aliquots and store at ≤ -20°C.
  Make any further dilutions of the reconstituted protein in appropriate buffered solution containing a carrier protein, such as 0.1% HSA or BSA.

• Storage:
  The lyophilized protein is stable at room temperature for 3 weeks.
  Store at -20°C, preferably desiccated.
  Upon reconstitution, apportion into working aliquots and store at 4°C for 2–7 days, or at -20°C.
  Avoid repeated freeze-thaw cycles.

Target Information

Noggin은 신경관 융합 및 관절 형성과 같은 다양한 발달 과정에 관여합니다. 기관의 형태형성은 상피 줄기세포층에서의 하향 성장으로 시작되며, 이는 WNT3A 단백질에 의한 β-catenin 안정화와 Noggin의 BMP 억제 신호를 통해 조절됩니다. Noggin 유전자 변이는 proximal symphalangism (SYM1) 및 multiple synostoses syndrome (SYNS1)과 관련이 있으며, 이들 질환은 여러 관절 융합을 특징으로 합니다.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.