Thermo Fisher Scientific Human Noggin Recombinant Protein
Human Noggin 재조합 단백질로, E. coli 발현 시스템에서 생산되며 >95% 순도와 낮은 엔도톡신 함량을 가집니다. WB, IHC, ELISA 등 다양한 실험에 사용 가능하며, BMP-4에 의한 알칼리성 포스파타제 생성 억제 활성으로 검증되었습니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- PHC1506
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Functional Assay (Functional): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC |
| Molecular Weight | 46.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 < 3 ng/mL; determined by the dose-dependent inhibition of alkaline phosphatase production induced by BMP-4 (5 ng/mL) in ATDC-5 chondrogenic cells. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom.
Reconstitute the lyophilized protein in 10 mM acetic acid to a concentration of 0.1–1.0 mg/mL.
Apportion the reconstituted protein into working aliquots and store at ≤ -20°C.
Make any further dilutions of the reconstituted protein in appropriate buffered solution containing a carrier protein, such as 0.1% HSA or BSA.Storage:
The lyophilized protein is stable at room temperature for 3 weeks.
Store at -20°C, preferably desiccated.
Upon reconstitution, apportion into working aliquots and store at 4°C for 2–7 days, or at -20°C.
Avoid repeated freeze-thaw cycles.
Target Information
Noggin은 신경관 융합 및 관절 형성과 같은 다양한 발달 과정에 관여합니다. 기관의 형태형성은 상피 줄기세포층에서의 하향 성장으로 시작되며, 이는 WNT3A 단백질에 의한 β-catenin 안정화와 Noggin의 BMP 억제 신호를 통해 조절됩니다. Noggin 유전자 변이는 proximal symphalangism (SYM1) 및 multiple synostoses syndrome (SYNS1)과 관련이 있으며, 이들 질환은 여러 관절 융합을 특징으로 합니다.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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