Thermo Fisher Scientific SSEA1 Recombinant Rabbit Monoclonal Antibody (PD00-42)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:1,000

Flow Cytometry (Flow)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Positive control: Human breast carcinoma tissue
• Predicted band size: 59 kDa
• Subcellular Location: Golgi apparatus, Golgi stack membrane

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system, or various tumor cells.
There are several variants of Lewis X, such as sialyl-Lewis X or sulphated Lewis X.
Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation based on calcium-dependent Le(x)-Le(x) interaction. This process is involved, for example, in embryo compaction or in autoaggregation of teratocarcinoma cells.
Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins.
CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.