Thermo Fisher Scientific RT1.B Monoclonal Antibody (OX-6)
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Rat MHC Class II RT1B 항원을 인식하는 OX-6 클론 단일클론 항체로, 마우스 유래 IgG1 형식입니다. IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, Protein G로 정제된 고순도 액상 형태입니다. 항원 회복 시 sodium citrate buffer(pH 6.0) 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX-6 |
| Immunogen | Rat thymocyte membrane glycoprotein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_934682 |
Product Specific Information
- Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for staining of paraffin sections.
- PLP fixation is recommended for optimal results.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Mouse anti-Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells.
Target Information
Gene complex that may play a role in antigen presentation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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