Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (OX-8), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat-1:10

View 1 publication 1 publication

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OX-8

Immunogen

Rat thymocyte membrane glycoproteins. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P07725-1,ncbiNodeId:10114,antigenRange:1-236,antigenLength:236,antigenImageFileName:MA1-80658_CD8_P07725-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80658_CD8_P07725-1_House_mouse_PDP.jpeg,sortOrder:4},{targetFamily:CD8,uniProtId:P05541-1,ncbiNodeId:10114,antigenRange:1-208,antigenLength:208,antigenImageFileName:MA1-80658_CD8_P05541-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80658_CD8_P05541-1_House_mouse_PDP.jpeg,sortOrder:7}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929597

Product Specific Information

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.