Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), PE, eBioscience

Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 450
• eFluor 660
• FITC
• Request custom conjugation

Product Specific Information

The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2).
CD107b is a highly glycosylated, type I transmembrane protein (~105 kDa), expressed in lysosomal/endosomal membranes of nearly all cells. It is also found on the surface of degranulating T cells, activated platelets, and some cancer cells.
Mutations in CD107b cause Danon disease, a lysosomal glycogen storage disorder.

Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and tested using Jurkat cells and the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Refer to BestProtocols®: Protocol A for intracellular proteins.
Recommended usage: 5 µL (0.5 µg) per test, typically 10⁵–10⁸ cells/test.

Spectral Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b)

• Member of lysosomal membrane glycoprotein family
• Provides selectins with carbohydrate ligands
• Involved in tumor cell metastasis, lysosome protection, and adhesion
• Precursor polypeptide: ~46 kDa; Mature form: 100–130 kDa (glycosylated)
• Structure: large amino-terminal intra-lysosomal domain, hydrophobic transmembrane domain, short cytoplasmic tail
• Monitors chaperone-mediated autophagy
• Exists as 3 variants: LAMP-2A, LAMP-2B, LAMP-2C
• Works with CD107a/LAMP-1 in lysosomal fusion and phagosomal maturation
• LAMP2 deficiency leads to autophagosome accumulation and Danon’s disease

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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