Thermo Fisher Scientific Tau Monoclonal Antibody (T46)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody recognizes an epitope near the C-terminal region (404–441 aa) of human Tau.
It reacts with all seven isoforms of human Tau (both binding-competent and binding-incompetent), A68 (an abnormal phosphorylated derivative of Tau), bovine Tau, and human NeuroFibrillary Tangles (NFTs).

Reactivity is independent of the phosphorylation state of Tau. Some cross-reactivity with MAP2 is also observed.
This antibody is suitable for immunohistochemical staining of Bouin’s-fixed or alcohol-fixed, paraffin-embedded, or frozen tissue sections.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. Its primary function is to promote tubulin polymerization and stabilize microtubules.

• The C-terminus binds axonal microtubules, while the N-terminus interacts with neural plasma membrane components, acting as a linker between both structures.
• Axonal polarity is determined by Tau/MAPT localization within the neuronal cell body.
• Short isoforms provide cytoskeletal plasticity, while longer isoforms contribute to stabilization.
• In its hyper-phosphorylated form, Tau constitutes paired helical filaments (PHF), the main component of neurofibrillary lesions in Alzheimer’s disease (AD).
• Hyper-phosphorylation disrupts Tau’s microtubule-binding function, leading to cytoskeletal destabilization and neuronal degeneration.
• Multiple kinases phosphorylate Tau, including GSK-3β, PKA, cdk5, and casein kinase II.
• Hyper-phosphorylated Tau is found in neurofibrillary lesions associated with Alzheimer’s disease, Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.