Thermo Fisher Scientific Phosphotyrosine Antibody Cocktail, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Product Specifications

Published species

Chemical, Human

Host/Isotype

Mouse / IgG1/IgG2b

Class

Cocktail

Type

Antibody

Clone

PY-7E1, PY20

Immunogen

Tyrosine-phosphorylated proteins.

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533027

Product Specific Information

13-6200 contains a cocktail of monoclonal mouse Phosphotyrosine containing clone PY-7E1, isotype IgG1, kappa and clone PY20, isotype IgG2b.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.