Thermo Fisher Scientific ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
ERK1 및 ERK2 단백질을 강하게 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 고순도 친화 크로마토그래피 정제, PBS 버퍼 및 sodium azide 함유, 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 42 publications |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 4 publications |
| ELISA | 0.1–1 mg/mL | - |
| Immunoprecipitation (IP) | 2–5 µg | 1 publication |
| Gel Shift (GS) | Assay-dependent | 1 publication |
| Miscellaneous PubMed (Misc) | - | 4 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Hamster, Human, Mouse, Rat, Xenopus |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ERK-7D8 |
| Immunogen | Synthetic peptide (aa 324–345) corresponding to a segment near the C-terminus of rat ERK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533024 |
Product Specific Information
This antibody reacts strongly with both ERK1 and ERK2. Reactivity has been confirmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain, and recombinant ERK2.
Target Information
ERK1 and ERK2 are widely expressed and involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Activation occurs via growth factors, cytokines, viral infection, and G protein-coupled receptor ligands. Once activated, the ERK pathway phosphorylates cytoplasmic targets or translocates to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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