Thermo Fisher Scientific ERK1/ERK2 Monoclonal Antibody (ERK-7D8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 42 publications 42 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1 µg/mL

View 4 publications 4 publications

ELISA (ELISA)

0.1-1 mg/mL

-

Immunoprecipitation (IP)

2-5 µg

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat, Xenopus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ERK-7D8

Immunogen

Synthetic peptide (aa 324-345) corresponding to a segment near the C-terminus of rat ERK1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERK1/2,uniProtId:P63086-1,ncbiNodeId:10114,antigenRange:324-345,antigenLength:358,antigenImageFileName:13-6200_ERK12_P63086-1_House_mouse.svg,antigenImageFileNamePDP:13-6200_ERK12_P63086-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533024

Product Specific Information

This antibody reacts strongly with both ERK1 and ERK2. Reactivity has been confirmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain, and recombinant ERK2.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.