Thermo Fisher Scientific Phospho-Vinculin (Tyr822) Polyclonal Antibody
Phospho-Vinculin (Tyr822) 특이적 인식 항체로, 인간·마우스·랫트 시료에서 사용 가능. Western blot, IHC, ICC/IF에 적용. 액상 형태, 고순도 정제 및 안정적 저장 (-20°C). 세포 접착 및 골격 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human VCL (Accession P18206), corresponding to amino acid residues around phosphorylated Tyr822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816299 |
Product Specific Information
This antibody detects endogenous levels of Vinculin only when phosphorylated at Tyrosine 822.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques: the beta subunit of integrin binds to talin, talin binds to vinculin, and vinculin interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds and cross-links actin filaments, vinculin is a key element in linking the extracellular matrix to the cytoplasmic microfilament system. Mutations in this gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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