Thermo Fisher Scientific CD63 Monoclonal Antibody (NVG-2), APC, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: -
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• View 3 publications

Product Specifications

Additional Formats

• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody NVG-2 reacts with mouse CD63, also known as Lysosomal-Associated Membrane Protein 3 (LAMP-3) or tetraspanin 30 (TSPN30), a member of the tetraspanin family characterized by four transmembrane domains. CD63 is expressed on various hematopoietic cells such as granulocytes, B lymphocytes, and platelets, as well as non-hematopoietic cells. It is found on the cell membrane, late endocytic vesicles, lysosomes, exosomes, and specialized granules.

On the cell surface, CD63 interacts with proteins forming tetraspanin-enriched microdomains (TEM). Its high expression may indicate cell activation; thus, CD63 is used as an activation marker for basophils, platelets, and other cells.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining of mouse peritoneal exudate cells using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended usage ≤ 0.25 µg per test (100 µL final volume). Cell number may range from 10^5–10^8 cells/test. Titrate for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD63 (LAMP-3) is a glycoprotein of the tetraspanin family found in late endosomes, lysosomes, and secretory vesicles. It is also present on the plasma membrane, often following cell activation, making it a useful basophil activation marker. CD63 interacts with integrins, affects phagocytosis and migration, and is involved in H/K-ATPase trafficking and ROMK1 channel regulation. It also serves as a T-cell costimulation molecule and can be used for carcinoma prognosis.

CD63 is expressed on activated platelets and translocated to the plasma membrane after activation. It is also found in monocytes, macrophages, and weakly in granulocytes, B, and T cells. CD63 is identical to melanoma-associated antigen ME491 and platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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