Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (OX26), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 1 publication

Product Specifications

Product Specific Information

The monoclonal antibody OX26 reacts with rat CD71 (Transferrin Receptor), a glycoprotein functioning as a homodimer on cell surfaces to mediate iron uptake via binding to iron-saturated transferrin.
CD71 is expressed by proliferating hematopoietic lineage cells, but not by mature resting cells or hematopoietic stem cells. Expression increases upon antigen or mitogen stimulation, correlating with cell proliferation.
It is also expressed in endothelial cells of brain capillaries, cortical neurons, oligodendrocytes, kidney tubules, hepatocytes, pancreatic cells, and muscle cells.

The OX26 antibody has been reported to exhibit agonist activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on rat bone marrow cells at ≤0.25 µg/test (100 µL final volume).
  • Recommended to titrate for optimal performance.
  • Typical cell number: 10⁵–10⁸ cells/test.
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release, and the apotransferrin-receptor complex is recycled to the cell surface.
The transferrin receptor is essential for erythrocyte and nervous system development. Serum transferrin receptor (sTfR) is used to detect erythropoietin (EPO) misuse and to diagnose anemias related to conditions such as rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV infection.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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