Thermo Fisher Scientific MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7, 1468)
Recombinant mouse monoclonal antibody against MCM7 protein for WB, IHC(P), and peptide array applications. Reacts with human and dog samples. Expressed in HEK293 cells, purified via Protein A/G, and supplied as a liquid at 200 µg/mL. For research use o...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMCM7, 1468 |
| Immunogen | Recombinant human MCM7 protein fragment (aa195–319) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance (MCM) proteins essential for the initiation of eukaryotic genome replication. The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may participate in replication fork formation and recruitment of other DNA replication-related proteins.
The MCM complex containing MCM2, 4, 6, and 7 possesses DNA helicase activity and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase CDK4 associates with MCM7 and may regulate its interaction with the tumor suppressor protein RB1/RB.
Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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