Thermo Fisher Scientific HDAC1 Monoclonal Antibody (10E2)
HDAC1 단백질을 인식하는 Mouse monoclonal antibody (clone 10E2). Western blot, IHC, ICC/IF, Flow, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein G 정제, 1 mg/mL 농도, PBS에 0.1% sodium azide 함유. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Condition |
|---|---|
| Western Blot (WB) | 1:500–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP assay (ChIP) | 2.5 µg / 10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10E2 |
| Immunogen | Synthetic peptide: EEKPEAKGVKEEVKLA, corresponding to C-terminal amino acids 467–482 of Human and Mouse HDAC1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539454 |
Additional Formats
Product Specific Information
This target has a predicted molecular weight of 55 kDa. Actual Western blot results show a band at approximately 57 kDa.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression.
HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex.
It interacts with the retinoblastoma tumor-suppressor protein, forming a complex that regulates cell proliferation and differentiation.
Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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