Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (12A380)
CD254 (RANK Ligand) 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 인간 및 생쥐 반응성이 있으며, 단백질 G로 정제된 비결합 항체입니다. RANKL 관련 신호전달 및 골흡수 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 4–6 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:250 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:10–1:500 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:10–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A380 |
| Immunogen | Bacterially expressed fusion protein containing residues 1–317 of mouse RANK ligand |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1087030 |
Product Specific Information
Suggested positive control: activated T cells or RAW cells, antigen standard for TNFSF11 (transient overexpression lysate).
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family, which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses. Activation of T cells induces expression of this gene, leading to increased osteoclastogenesis and bone loss. RANKL activates antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and TRAF6, suggesting a role in cell apoptosis regulation. Gene disruption in mice leads to severe osteopetrosis, lack of osteoclasts, and defects in T/B lymphocyte differentiation and mammary gland development. Two alternatively spliced transcript variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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