Thermo Fisher Scientific ROCK1 (Cleaved Asp1113) Monoclonal Antibody (154C1465)
ROCK1 단백질의 절단형(Cleaved Asp1113)을 특이적으로 인식하는 단일클론 항체로, 세포자멸사(apoptosis) 연구에 활용 가능. Western blot 및 면역세포염색(ICC/IF)에 사용. Caspase-3에 의해 절단된 ROCK1 검출에 적합. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rabbit, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 154C1465 |
| Immunogen | Synthetic peptide corresponding to residues G(1114) N L P E S R I E G W L S(1126) of human ROCK-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2182003 |
Product Specific Information
This monoclonal antibody recognizes the cleaved C-terminus of ROCK-1 and does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this antibody can be used for the detection of apoptosis.
A suggested positive control is Jurkat cells treated with camptothecin or anti-Fas antibody to induce apoptosis.
Target Information
Rho-associated coiled coil-containing protein kinase (ROCK)-1 is a substrate of caspase-3 and an effector of the small GTPase Rho. It contributes to myosin light chain (MLC) activation via direct phosphorylation and inhibition of MLC phosphatase.
During apoptosis, ROCK-1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage leads to phosphorylation of MLC and membrane blebbing, key events in the execution phase of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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