Thermo Fisher Scientific HTRA2 Monoclonal Antibody (196C429)
HTRA2 단백질을 인식하는 mouse monoclonal antibody로 Western blot 및 IHC(P) 실험에 적합. Human 시료 반응성. Protein G로 정제된 liquid 형태이며, 0.5 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 196C429 |
| Immunogen | Synthetic peptide corresponding to residues 335–350 of human Htra2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075687 |
Product Specific Information
Suggested positive control: HL-60 or Jurkat whole cell lysate.
Target Information
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein, called Omi/HtrA2, is a mitochondrial protein that binds to IAP. Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis, indicating that Omi could participate in the mitochondrial apoptotic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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