Thermo Fisher Scientific HTRA2 Monoclonal Antibody (196C429)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

196C429

Immunogen

Synthetic peptide corresponding to residues 335-350 of human Htra2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HTRA2,uniProtId:O43464-1,ncbiNodeId:9606,antigenRange:335-350,antigenLength:458,antigenImageFileName:MA1-41040_HTRA2_O43464-1_House_mouse.svg,antigenImageFileNamePDP:MA1-41040_HTRA2_O43464-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075687

Product Specific Information

Suggested positive control: HL-60 or Jurkat whole cell lysate.

Target Information

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.