Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser2) Recombinant Rabbit Monoclonal Antibody (JM11-51)
RNA polymerase II CTD Ser2 인산화 형태를 검출하는 recombinant rabbit monoclonal antibody. Western blot, IHC, ICC, Flow cytometry, ChIP 등 다양한 응용 가능. 높은 특이성과 재현성, 로트 간 일관성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:1000 |
| ChIP assay (ChIP) | 5 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM11-51 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser2 of Human POLR2A (aa 1590–1630) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809914 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
RNA polymerase II is responsible for synthesizing mRNA and many non-coding RNAs. The POLR2A gene encodes the largest subunit (RPB1) of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence regulate transcription cycle progression and the recruitment of RNA processing factors.
Phosphorylation at Serine-2 and Serine-5 residues within the CTD plays a key role in coordinating transcription elongation and RNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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