Thermo Fisher Scientific Bacteriophage T7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Enterobacteria phage T7 (Bacteriophage T7) 1 protein (710-805aa)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_3091698

Target Information

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an abortive phase where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.