Thermo Fisher Scientific SUP45 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508/S288c) (Bakers yeast) SUP45 protein (26-262aa) if (typeof window.$mangular ===undefined|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson =[{targetFamily:SUP45,uniProtId:P12385-1,ncbiNodeId:-23,antigenRange:26-262,antigenLength:437,antigenImageFileName:PA5-144559\_SUP45\_P12385-1\_Rabbit.svg,antigenImageFileNamePDP:PA5-144559\_SUP45\_P12385-1\_Rabbit\_PDP.jpeg,sortOrder:1}]; $mangular.isB2BCMGT = ``false``; $mangular.isEpitopesModalImageMultiSizeEnabled =true`;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_3092406

Target Information

Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed:7556078, PubMed:20947765, PubMed:34413231). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:7556078, PubMed:20947765). SUP45/eRF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed:7556078, PubMed:20947765, PubMed:34413231). Following GTP hydrolysis by SUP35/eRF3, SUP35/eRF3 dissociates, permitting SUP45/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed:7556078, PubMed:20947765, PubMed:34413231).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.