Thermo Fisher Scientific c-Myc Oncoprotein Monoclonal Antibody (rMYC909)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Does not react with Mouse, Rat, Chicken

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with cell proliferation and regulation.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development. Deregulated expression of c-Myc induces apoptosis in different cell types.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino or carboxy termini of target proteins.
Amplification of the c-Myc gene has been found in lung, breast, and colon carcinomas, while N-Myc amplification is observed in neuroblastomas. Translocation of the c-Myc locus to immunoglobulin loci is described in Burkitt’s lymphoma and other B-cell lymphoproliferative conditions.
Aberrant c-Myc expression occurs in tumors of diverse origins including colorectal, gastric, hepatic, mammary, ovarian, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.