Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells. Crosslinking via immobilized UCHT1 triggers intracellular signaling leading to activation and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg) per test.
Instrument Compatibility: BV711 emits at 713 nm, suitable for cytometers with a violet (405 nm) laser.

Staining Recommendations:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Light Sensitivity: Protect vial and samples from light.
Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
Compatibility: Not suitable for methanol-based fixation.

Fluorophore Properties:

• Excitation: 407 nm
• Emission: 713 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all members of the immunoglobulin superfamily located on chromosome 11. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. It plays roles in TCR-induced growth arrest, survival, and proliferation. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for diagnostic use or resale without authorization.

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