Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450–660)
• FITC
• Functional Grade
• NovaFluor series (Blue, Red, Violet, Yellow)
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP series
• Super Bright series (436–780)
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers. CD8 interacts with MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; use ≤ 0.5 µg/test (100 µL volume). Optimal concentration should be titrated empirically.

Brilliant Violet™ 711 (BV711) emits at 713 nm and is designed for cytometers with a 405 nm violet laser.

Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dye-conjugated antibodies.
• Protect from light due to photo-induced oxidation sensitivity.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54). Store fixed samples up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD8 is a type I transmembrane glycoprotein critical for T cell signal transduction, differentiation, and activation. It forms heterodimers (CD8αβ) on T cells and homodimers (CD8αα) on subsets of T cells, NK cells, monocytes, and dendritic cells. CD8 binds MHC-I molecules, facilitating recruitment of Lck kinase and activation of cytotoxic T lymphocytes (CTLs). CTL differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지