Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 711, eBioscience
마우스 CD8a 항원을 인식하는 53-6.7 클론 단일클론 항체로, Brilliant Violet 711 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.5 µg/test 농도로 사용 가능합니다. 405 nm 레이저에 적합하며, 빛에 민감하므로 암조건에서 보관해야 합니다.
- 카탈로그번호
- 407-0081-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 711, eBioscience
Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 711, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2921028 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor series (450–660)
- FITC
- Functional Grade
- NovaFluor series (Blue, Red, Violet, Yellow)
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP series
- Super Bright series (436–780)
- Request custom conjugation
Product Specific Information
The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers. CD8 interacts with MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; use ≤ 0.5 µg/test (100 µL volume). Optimal concentration should be titrated empirically.
Brilliant Violet™ 711 (BV711) emits at 713 nm and is designed for cytometers with a 405 nm violet laser.
Recommendations:
- Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dye-conjugated antibodies.
- Protect from light due to photo-induced oxidation sensitivity.
- Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54). Store fixed samples up to 3 days at 4°C in the dark.
- Not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.
Target Information
CD8 is a type I transmembrane glycoprotein critical for T cell signal transduction, differentiation, and activation. It forms heterodimers (CD8αβ) on T cells and homodimers (CD8αα) on subsets of T cells, NK cells, monocytes, and dendritic cells. CD8 binds MHC-I molecules, facilitating recruitment of Lck kinase and activation of cytotoxic T lymphocytes (CTLs). CTL differentiation is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.