Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 711

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue and Yellow series
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The RM4-5 monoclonal antibody reacts with mouse CD4, a 55 kDa cell surface receptor expressed on thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is critical for T cell development and function. RM4-5 binding is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤ 0.25 µg/test
• Recommended: Titrate for optimal performance
• Fluorophore: Brilliant Violet™ 711 emits at 713 nm (violet laser, 405 nm excitation)

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect vial and samples from light.
Fixation: Compatible with IC Fixation Buffer (00-8222-49) or Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C.
Not Compatible: Methanol-based fixation.
Laser: Violet (407 nm excitation, 713 nm emission)

Brilliant Violet™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for MHC class II recognition and HIV binding. Expressed mainly in T helper cells, and also in monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and other tissues. CD4 participates in T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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