Thermo Fisher Scientific eIF3b Recombinant Rabbit Monoclonal Antibody (JE54-00)
eIF3b 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE54-00 |
| Immunogen | Recombinant protein within Human eIF3B (aa 81–220) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2890441 |
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, required for multiple steps in initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates formation of the 43S pre-initiation complex (43S PIC). It stimulates mRNA recruitment and scanning for AUG recognition, and is essential for ribosomal recycling and prevention of premature subunit joining. The complex selectively regulates translation of mRNAs involved in cell proliferation, differentiation, and apoptosis through distinct RNA stem-loop binding mechanisms. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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