Thermo Fisher Scientific Calponin 1 Monoclonal Antibody (1H5B5)
Calponin 1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ELISA 등에 사용 가능. 인간 시료에 반응하며, 단백질 G로 정제된 액상 형태. 연구용으로 근육 수축 관련 단백질 분석에 적합.
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Thermo Fisher Scientific Calponin 1 Monoclonal Antibody (1H5B5)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H5B5 |
| Immunogen | Purified recombinant fragment of human CNN1 (aa 16–165) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898414 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Calponin is a thin filament-associated protein involved in the regulation and modulation of smooth muscle contraction. It binds to actin, calmodulin, troponin C, and tropomyosin. Calponin is expressed in smooth muscle and tissues with significant smooth muscle content. Two isoforms exist (34 kDa and 29 kDa); the 29 kDa form is mainly found in the urogenital tract. Calponin expression is also observed in myoepithelial cells from benign and malignant breast lesions. It stains smooth muscle, myoepithelial cells, myofibroblasts, keratinocytes, and nerve fibers, aiding differentiation of breast collagenous spherulosis (positive) from adenoid cystic carcinoma. Adenoid cystic carcinoma in salivary gland tumors is calponin positive.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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