Thermo Fisher Scientific IKZF3 Monoclonal Antibody (OTI4H7), TrueMAB
IKZF3 단백질을 인식하는 마우스 단클론 항체로, Western blot과 IHC(P)에서 검증됨. 인간 시료 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. B세포 발달 및 전사 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4H7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–301 of human IKZF3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The IKZF3 gene encodes a member of the Ikaros family of zinc-finger proteins.
Ikaros, Aiolos, and Helios are hematopoietic-specific transcription factors regulating lymphocyte development.
IKZF3 (Aiolos) is important in B lymphocyte proliferation and differentiation, and can participate in chromatin remodeling.
Regulation of gene expression in B lymphocytes by Aiolos involves the formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers.
At least six alternative transcripts encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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